Immunochromogenic Reagent (EN)

Designed for detecting common mouse/rabbit-derived primary antibodies, the kit includes an inhibitor to block endogenous peroxidase in tissue sections.

The primary antibody selectively binds to the target antigen, forming an antigen-antibody complex. Following primary antibody application, the reagent reduces non-specific binding and improves affinity.

The ultra-sensitive enzyme-labeled anti-mouse/rabbit IgG polymer (with HRP) recognizes the primary antibody in the complex.

DAB Solution A and B are mixed to prepare the chromogenic solution, which HRP catalyzes to produce brown staining at the antigen location.

An enhancer amplifies the brown signal, while hematoxylin provides nuclear counterstaining.